PD62-10 THE ROLE OF MTOR IN DIFFERENTIATION OF SPERMATOZOA

You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology (PD62)1 Sep 2021PD62-10 THE ROLE OF MTOR IN DIFFERENTIATION SPERMATOZOA Karen Doersch, Kadijah Abston, Hannah Kim, Amanda Pereira, Xiaoyang Su, and Xin Zhiguo Li DoerschKaren Doersch More articles by this author , AbstonKadijah Abston KimHannah Kim PereiraAmanda Pereira SuXiaoyang Su LiXin View All Author Informationhttps://doi.org/10.1097/JU.0000000000002099.10AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The mammalian target rapamycin (mTOR) is an evolutionarily preserved protein that regulates homeostatic processes, including growth-factor response, autophagy, cytoskeletal organization, metabolism. Rapamycin, inhibitor mTOR, well-established extend organisms’ lifespan from yeast mice; however, its application in humans causes male infertility. Because mTOR pathway function germ cells not well-understood, the objective study was examine role cell development. METHODS: A testis-specific mTor-conditional knockout (CKO) mouse created using a lox-cre system driven Neurog3-cre, which expressed late spermatogenesis. Testis were examined H&E, electron microscopy, immunofluorescence microscopy. RNA-seq small also used. Metabolomic analysis testis tissue with mass spectroscopy performed. RESULTS: CKO mutants are completely infertile arrest spermiogenesis at round spermatid stage on H&E demonstrated decreases markers mature spermatozoa compared controls, corroborating finding spermatid. Furthermore, micro-RNAs (miRNAs) aberrant miRNA expression, may play halting spermatic increased amino acids, such as threonine, glutamine, isoleucine, controls. had altered nucleic acid levels, elevated guanosine but decreased acid-monophosphate species. Aberrant likely represents alterations guanosine-triphosphate-dependent signaling via mTORC1 while nucleotide monophosphates represent energy production translation. CONCLUSIONS: This demonstrates specific leads phase. mRNA transcription translation CKO. Our results demonstrate direct for mTOR-mediated regulation differentiation Source Funding: None © 2021 American Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e1076-e1076 Advertisement Copyright Permissions© Inc.MetricsAuthor Information Expand Loading ...